Protein solid-state NMR resonance assignments from (13C,13C) correlation spectroscopy
نویسندگان
چکیده
منابع مشابه
Protein solid-state NMR resonance assignments from (C,C) correlation spectroscopy
Magic angle spinning (MAS) solid-state NMR is rapidly developing into a tool to study biomolecular 3D structure, protein aggregation and ligand-binding to membrane receptors. For many structural studies, near-complete sequential resonance assignments are a prerequisite and have now been obtained for several (membrane) proteins. MAS-based spectral assignment methods have thus far relied on a col...
متن کاملAutomated Protein NMR Resonance Assignments
NMR resonance peak assignment is one of the key steps in solving an NMR protein structure. The assignment process links resonance peaks to individual residues of the target protein sequence, providing the prerequisite for establishing intra- and inter-residue spatial relationships between atoms. The assignment process is tedious and time-consuming, which could take many weeks. Though there exis...
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A number of arylamides have been synthesized and found to exhibit potent antimicrobial activities against a broad spectrum of Gram-positive and Gram-negative bacteria while exhibiting low toxicity toward eukaryotic cells. These facially amphiphilic foldamers have a relatively rigid intramolecular hydrogen-bonded arylamide as a framework, which places trifluormethyl versus positively charged ami...
متن کاملConstant-time through-bond 13C correlation spectroscopy for assigning protein resonances with solid-state NMR spectroscopy.
Even as available magnetic fields for NMR continue to increase, resolution remains one of the most critical limitations in assigning and solving structures of larger biomolecules. Here we present a novel constant-time through-bond correlation spectroscopy for solids that offers superior resolution for 13C chemical shift assignments in proteins. In this experiment, the indirect evolution and tra...
متن کامل2H-DNP-enhanced 2H-13C solid-state NMR correlation spectroscopy.
Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution (2)H-(13)C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved (2)H-(13)C correlation spectra with a signal e...
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ژورنال
عنوان ژورنال: Physical Chemistry Chemical Physics
سال: 2004
ISSN: 1463-9076,1463-9084
DOI: 10.1039/b411689e